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The client system should work on the client s current computer network, which is connected to the Internet and has Windows XP or better. The system should be easy to install. These statements need to be re ned into a more precise statement in order to make them testable. What, for example, does easy to install mean We will look at this in the next chapter. From each story that we have discussed with the client, we extract the key functional details. These are grouped in sections with other story lines that are clearly related. These requirements are categorized on the basis of which are mandatory, desirable, and optional. To do this, we need to have an estimate of the time we might take to complete these, and this will help us to make these decisions. The next section looks at the process of trying to estimate this. Naturally, we must consult the client on which he or she thinks are mandatory and so forth. We have to be realistic, and you must not promise to do more than you can achieve in the time given.

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A ferrocene species, used to regenerate the oxidized form of GO and deliver electrons to the electrode surface (n 2 mol electrons/mol glucose), shows very fast kinetics for both processes, so that Eq 713 is valid (a) Estimate the current, in microamperes, that would be obtained for a 6-mM solution of glucose (this is the normal physiological concentration in whole blood) (b) Estimate the current that would be obtained if the reaction layer thickness was reduced by one order of magnitude (ie, to 0010 mm) 2 The mathematical descriptions of enzyme-based biosensor response assume that steady-state concentrations of substrates and products exist within the thin reaction layer at the surface of the transducer, and that the bulk concentrations of these species do not change signi cantly with time Suggest one experimental situation in which these assumptions are not expected to be valid 3.

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private static long factorialLookup(int value){ return factorials[value-1]; }

If we have a model like the one above, we can use something like the function point method and object point method. Here we try to estimate the amount of effort required to build a story or a screen with its accompanying functionality.

Polystyrene optical bers can be derivatized by rst nitrating and then reducing the surface to produce aminophenyl groups These can then be diazotized to allow protein immobilization through (mainly) tyrosine residues An evanescentwave uorescence biosensor for glucose is to be designed, using a bienzyme system consisting of hexokinase and glucose-6-phosphate dehydrogenase (both are <10 nm in hydrodynamic diameter) according to the following reactions: Glucose ATP G6P ADP ! G6P NADP 6-Phosphoglyceric acid NADPH ! where the product NADPH uoresces at 460 nm following excitation at 340 nm Which of the following three sensor con gurations may be expected to generate.

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To do this, we look at each story and consider the functions contained in it. We then try to categorize what sort of function this is. We can nd information that estimates the amount of effort each category of function might require; this data is collected in industrial organizations, and some of it is published. We include some examples here. It is a good practice to try to measure your own efforts for these functions to see if they agree with the estimates and to inform future projects.

the largest uorescence intensity signals for the same bulk glucose concentration (a) Simultaneous coadsorption of both enzymes to underivatized polystyrene. (b) Nonpolymerizing covalent immobilization of glucose-6-phosphate dehydrogenase, followed by cross-linked immobilization of hexokinase. (c) Coimmobilization of the two enzymes through tyrosine residues to form a bienzyme monolayer on the polystyrene surface. 4. Why is the operational stability of an enzyme-based thermal sensor for oxalic acid expected to be poorer than an amperometric oxalate sensor prepared using the same enzyme, oxalate oxidase

These are called by the doFactorialLookup() wrapper. This rst populates the array by calling the earlier factorial() method (this could be done much more ef ciently, but the startup time is insigni cant), then repeatedly calls factorialLookup():

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